Biophysics, Spectroscopy and Spectrometry
Understanding the nature of a biomolecule requires understanding its structure and function within its native environment under a range of conditions.
Intense research is being carried out at the Franklin on several analytical tools. New mass spectrometer designs are being constructed and optimised to improve sensitivity and comprehensivity. Superior designs of laser-based spectroscopies such as Raman are being developed to improve detection and classification. NMR approaches involving both pulse sequences and data processing are being developed to allow detection of dynamic (in cellulo) biomolecule interactions as well as identify modes of action of drugs. These approaches dovetail with our research into new chemistries and chemical biology. Not only to enhance the fidelity of our data but to also allow the techniques themselves to be improved. Femtosecond transient absorption and electron paramagnetic resonance (EPR) spectroscopy, for example, is used to investigate the mechanisms of radical formation. NMR and MS provide insight into selectivity and clues for better synthesis control. These insights will allow us to optimize reaction conditions and improve selectivity.
